1.Answer these questions about cultivation technique:
a) Why are media and cultures in petri plates stored upside down?
b) Why is it necessary to heat a loop to redness before using it to transfer bacteria?
c) Why is it necessary to cool a loop after it has been sterilized before placing it into a bacterial culture?
2.Answer these questions about making smears for staining:
a) What are three qualities of an effective and useful bacterial smear?
b) What would happen to the smear if you failed to “heat fix” the slide before staining?
3.a) Review the lab manual Where are Microbes and list below all the environments you will sample. For those where you have some options, list environments you would be curious to sample.
b) Are you expecting the same result on all 8 environment sample plates. Why or why not – explain your answer for full credit.
4. What is the difference between results and conclusions? Why do scientist have to include both in thier reports.
5. Should scientists write down observation on loose scraps of paper? Why or Why not?